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Protein

Mitochondrial import inner membrane translocase subunit TIM50

Gene

TIM50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity.3 Publications

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. mitochondrion targeting sequence binding Source: SGD

GO - Biological processi

  1. protein import into mitochondrial matrix Source: SGD
  2. regulation of mitochondrial membrane permeability Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33972-MONOMER.
ReactomeiREACT_334110. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene namesi
Name:TIM50
Ordered Locus Names:YPL063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPL063w.
SGDiS000005984. TIM50.

Subcellular locationi

Mitochondrion inner membrane 3 Publications; Single-pass membrane protein 3 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini44 – 11471Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei115 – 13117HelicalSequence AnalysisAdd
BLAST
Topological domaini132 – 476345Mitochondrial intermembraneSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: Reactome
  3. mitochondrial inner membrane presequence translocase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4343Mitochondrion2 PublicationsAdd
BLAST
Chaini44 – 476433Mitochondrial import inner membrane translocase subunit TIM50PRO_0000043139Add
BLAST

Proteomic databases

MaxQBiQ02776.
PaxDbiQ02776.
PeptideAtlasiQ02776.

Expressioni

Gene expression databases

GenevestigatoriQ02776.

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with preproteins in transit.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-30302,EBI-30302
Aldh2P118843EBI-30302,EBI-916402From a different organism.
oliP008425EBI-30302,EBI-9084292From a different organism.
TIM21P5322010EBI-30302,EBI-23128
TIM23P3289712EBI-30302,EBI-9136

Protein-protein interaction databases

BioGridi36116. 28 interactions.
DIPiDIP-8544N.
IntActiQ02776. 21 interactions.
MINTiMINT-3374965.
STRINGi4932.YPL063W.

Structurei

3D structure databases

ProteinModelPortaliQ02776.
SMRiQ02776. Positions 195-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini187 – 330144FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Domaini

The mitochondrial intermembrane region is required for generation but not stabilization of the TIM23 complex.1 Publication
The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity.1 Publication

Sequence similaritiesi

Belongs to the TIM50 family.Curated
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00550000075036.
HOGENOMiHOG000183658.
InParanoidiQ02776.
KOiK17496.
OMAiGYLSQYY.
OrthoDBiEOG7VDZ0W.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR004274. NIF.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q02776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD
60 70 80 90 100
KPKSILTDDM LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK
110 120 130 140 150
RQTSTDIKRE KYANWFYIFS LSALTGTAIY MARDWEPQES EELKKDIDNG
160 170 180 190 200
YTLSLMYKRF KARFNSMFTY FQEPPFPDLL PPPPPPPYQR PLTLVITLED
210 220 230 240 250
FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN YMMYSDKIAE
260 270 280 290 300
KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK
310 320 330 340 350
LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN
360 370 380 390 400
LAEEFDHRVK KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE
410 420 430 440 450
EGQKNYLMFM KMIEEEKEKI RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK
460 470
IQLEKQKEVD ALFEEEKKKK KIAESK
Length:476
Mass (Da):55,099
Last modified:October 31, 1996 - v1
Checksum:i778D54F5C4ED27C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA. Translation: AAB68302.1.
BK006949 Genomic DNA. Translation: DAA11368.1.
PIRiS60927.
RefSeqiNP_015262.1. NM_001183877.1.

Genome annotation databases

EnsemblFungiiYPL063W; YPL063W; YPL063W.
GeneIDi856042.
KEGGisce:YPL063W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39205 Genomic DNA. Translation: AAB68302.1.
BK006949 Genomic DNA. Translation: DAA11368.1.
PIRiS60927.
RefSeqiNP_015262.1. NM_001183877.1.

3D structure databases

ProteinModelPortaliQ02776.
SMRiQ02776. Positions 195-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36116. 28 interactions.
DIPiDIP-8544N.
IntActiQ02776. 21 interactions.
MINTiMINT-3374965.
STRINGi4932.YPL063W.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBiQ02776.
PaxDbiQ02776.
PeptideAtlasiQ02776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL063W; YPL063W; YPL063W.
GeneIDi856042.
KEGGisce:YPL063W.

Organism-specific databases

CYGDiYPL063w.
SGDiS000005984. TIM50.

Phylogenomic databases

eggNOGiCOG5190.
GeneTreeiENSGT00550000075036.
HOGENOMiHOG000183658.
InParanoidiQ02776.
KOiK17496.
OMAiGYLSQYY.
OrthoDBiEOG7VDZ0W.

Enzyme and pathway databases

BioCyciYEAST:G3O-33972-MONOMER.
ReactomeiREACT_334110. Mitochondrial protein import.

Miscellaneous databases

NextBioi980986.
PROiQ02776.

Gene expression databases

GenevestigatoriQ02776.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR004274. NIF.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel."
    Geissler A., Chacinska A., Truscott K.N., Wiedemann N., Brandner K., Sickmann A., Meyer H.E., Meisinger C., Pfanner N., Rehling P.
    Cell 111:507-518(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, TRANSIT PEPTIDE CLEAVAGE SITE, LACK OF PHOSPHATASE ACTIVITY, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23.
  4. "Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes."
    Yamamoto H., Esaki M., Kanamori T., Tamura Y., Nishikawa S., Endo T.
    Cell 111:519-528(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, TRANSIT PEPTIDE CLEAVAGE SITE, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23.
  5. "Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex."
    Chacinska A., Rehling P., Guiard B., Frazier A.E., Schulze-Specking A., Pfanner N., Voos W., Meisinger C.
    EMBO J. 22:5370-5381(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN INTERMEMBRANE.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
    Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
    Cell 120:817-829(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.

Entry informationi

Entry nameiTIM50_YEAST
AccessioniPrimary (citable) accession number: Q02776
Secondary accession number(s): D6W3V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2005
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.