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Q02776 (TIM50_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene names
Name:TIM50
Ordered Locus Names:YPL063W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity. Ref.3 Ref.4 Ref.5

Subunit structure

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with preproteins in transit. Ref.3 Ref.4 Ref.8

Subcellular location

Mitochondrion inner membrane; Single-pass membrane protein Ref.3 Ref.4 Ref.6.

Domain

The mitochondrial intermembrane region is required for generation but not stabilization of the TIM23 complex. Ref.5

The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity. Ref.5

Miscellaneous

Present with 2400 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TIM50 family.

Contains 1 FCP1 homology domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

oliP008425EBI-30302,EBI-9084292From a different organism.
TIM23P328973EBI-30302,EBI-9136

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion Probable
Chain44 – 476433Mitochondrial import inner membrane translocase subunit TIM50
PRO_0000043139

Regions

Topological domain44 – 11471Mitochondrial matrix Potential
Transmembrane115 – 13117Helical; Potential
Topological domain132 – 476345Mitochondrial intermembrane Potential
Domain187 – 330144FCP1 homology

Sequences

Sequence LengthMass (Da)Tools
Q02776 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 778D54F5C4ED27C8

FASTA47655,099
        10         20         30         40         50         60 
MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD KPKSILTDDM 

        70         80         90        100        110        120 
LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK RQTSTDIKRE KYANWFYIFS 

       130        140        150        160        170        180 
LSALTGTAIY MARDWEPQES EELKKDIDNG YTLSLMYKRF KARFNSMFTY FQEPPFPDLL 

       190        200        210        220        230        240 
PPPPPPPYQR PLTLVITLED FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN 

       250        260        270        280        290        300 
YMMYSDKIAE KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK 

       310        320        330        340        350        360 
LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN LAEEFDHRVK 

       370        380        390        400        410        420 
KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE EGQKNYLMFM KMIEEEKEKI 

       430        440        450        460        470 
RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK IQLEKQKEVD ALFEEEKKKK KIAESK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel."
Geissler A., Chacinska A., Truscott K.N., Wiedemann N., Brandner K., Sickmann A., Meyer H.E., Meisinger C., Pfanner N., Rehling P.
Cell 111:507-518(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, TRANSIT PEPTIDE CLEAVAGE SITE, LACK OF PHOSPHATASE ACTIVITY, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23.
[4]"Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes."
Yamamoto H., Esaki M., Kanamori T., Tamura Y., Nishikawa S., Endo T.
Cell 111:519-528(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, TRANSIT PEPTIDE CLEAVAGE SITE, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23.
[5]"Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex."
Chacinska A., Rehling P., Guiard B., Frazier A.E., Schulze-Specking A., Pfanner N., Voos W., Meisinger C.
EMBO J. 22:5370-5381(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAIN INTERMEMBRANE.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39205 Genomic DNA. Translation: AAB68302.1.
BK006949 Genomic DNA. Translation: DAA11368.1.
PIRS60927.
RefSeqNP_015262.1. NM_001183877.1.

3D structure databases

ProteinModelPortalQ02776.
SMRQ02776. Positions 195-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36116. 28 interactions.
DIPDIP-8544N.
IntActQ02776. 18 interactions.
MINTMINT-3374965.
STRING4932.YPL063W.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

PaxDbQ02776.
PeptideAtlasQ02776.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL063W; YPL063W; YPL063W.
GeneID856042.
KEGGsce:YPL063W.

Organism-specific databases

CYGDYPL063w.
SGDS000005984. TIM50.

Phylogenomic databases

eggNOGCOG5190.
GeneTreeENSGT00550000075036.
HOGENOMHOG000183658.
KOK17496.
OMAGYLSQYY.
OrthoDBEOG7VDZ0W.

Enzyme and pathway databases

BioCycYEAST:G3O-33972-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).
REACT_85873. Metabolism of proteins.

Gene expression databases

GenevestigatorQ02776.

Family and domain databases

Gene3D3.40.50.1000. 1 hit.
InterProIPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamPF03031. NIF. 1 hit.
[Graphical view]
SMARTSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
PROSITEPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980986.
PROQ02776.

Entry information

Entry nameTIM50_YEAST
AccessionPrimary (citable) accession number: Q02776
Secondary accession number(s): D6W3V2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families