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P53909

- ADE_YEAST

UniProt

P53909 - ADE_YEAST

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Protein
Adenine deaminase
Gene
AAH1, YNL141W, N1208, N1825
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism. Also exhibits a low activity towards N(6)-substituted adenines that are commonly known as the plant hormones cytokinins.4 Publications

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Kineticsi

  1. KM=55 µM for adenine1 Publication

pH dependencei

Optimum pH is 7.0.

Temperature dependencei

Optimum temperature is 30-37 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Zinc; catalytic By similarity
Metal bindingi18 – 181Zinc; catalytic By similarity
Metal bindingi204 – 2041Zinc; catalytic By similarity
Active sitei207 – 2071Proton donor By similarity
Sitei228 – 2281Important for catalytic activity By similarity
Metal bindingi285 – 2851Zinc; catalytic By similarity
Binding sitei286 – 2861Substrate By similarity

GO - Molecular functioni

  1. adenine deaminase activity Source: UniProtKB
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. adenine catabolic process Source: UniProtKB
  2. hypoxanthine salvage Source: SGD
  3. nucleotide metabolic process Source: UniProtKB-KW
  4. purine ribonucleoside monophosphate biosynthetic process Source: InterPro
  5. purine-containing compound salvage Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YNL141W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine deaminase (EC:3.5.4.2)
Short name:
ADE
Alternative name(s):
Adenine aminohydrolase
Short name:
AAH
Gene namesi
Name:AAH1
Ordered Locus Names:YNL141W
ORF Names:N1208, N1825
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL141w.
SGDiS000005085. AAH1.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691I → T in aah1-2; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication
Mutagenesisi72 – 721N → K in aah1-3; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication
Mutagenesisi219 – 2191D → G in aah1-4; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication
Mutagenesisi237 – 2371E → V in aah1-6; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication
Mutagenesisi329 – 3291K → E in aah1-7; impairs AAH1 degradation during postdiauxic growth. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Adenine deaminaseUniRule annotation
PRO_0000194360Add
BLAST

Post-translational modificationi

Probably ubiquitinated when cells enter quiescence in response to nutrient limitation, since it is specifically degraded via a process requiring the F-box protein SAF1 and components of the SKP1-Cullin-F-box complex.UniRule annotation

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiP53909.
PaxDbiP53909.
PeptideAtlasiP53909.

Expressioni

Inductioni

Reduced when grown in a poor nitrogen source medium and strongly down-regulated when cells enter quiescence under nutrient-limiting conditions.1 Publication

Gene expression databases

GenevestigatoriP53909.

Interactioni

Protein-protein interaction databases

BioGridi35686. 142 interactions.
DIPiDIP-4342N.
IntActiP53909. 4 interactions.
MINTiMINT-499127.
STRINGi4932.YNL141W.

Structurei

3D structure databases

ProteinModelPortaliP53909.
SMRiP53909. Positions 5-321.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1816.
GeneTreeiENSGT00730000111151.
KOiK01488.
OMAiMFRELYS.
OrthoDBiEOG7647CH.

Family and domain databases

HAMAPiMF_01962. Adenine_deaminase.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01430. aden_deam. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53909-1 [UniParc]FASTAAdd to Basket

« Hide

MVSVEFLQEL PKCEHHLHLE GTLEPDLLFP LAKRNDIILP EGFPKSVEEL    50
NEKYKKFRDL QDFLDYYYIG TNVLISEQDF FDLAWAYFKK VHKQGLVHAE 100
VFYDPQSHTS RGISIETVTK GFQRACDKAF SEFGITSKLI MCLLRHIEPE 150
ECLKTIEEAT PFIKDGTISA LGLDSAEKPF PPHLFVECYG KAASLNKDLK 200
LTAHAGEEGP AQFVSDALDL LQVTRIDHGI NSQYDEELLD RLSRDQTMLT 250
ICPLSNVKLQ VVQSVSELPL QKFLDRDVPF SLNSDDPAYF GGYILDVYTQ 300
VSKDFPHWDH ETWGRIAKNA IKGSWCDDKR KNGLLSRVDE VVTKYSH 347
Length:347
Mass (Da):39,635
Last modified:October 1, 1996 - v1
Checksum:iE25573A9F9EB7BB6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46843 Genomic DNA. Translation: CAA86885.1.
Z71417 Genomic DNA. Translation: CAA96024.1.
BK006947 Genomic DNA. Translation: DAA10407.1.
PIRiS55143.
RefSeqiNP_014258.1. NM_001182979.1.

Genome annotation databases

EnsemblFungiiYNL141W; YNL141W; YNL141W.
GeneIDi855581.
KEGGisce:YNL141W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46843 Genomic DNA. Translation: CAA86885.1 .
Z71417 Genomic DNA. Translation: CAA96024.1 .
BK006947 Genomic DNA. Translation: DAA10407.1 .
PIRi S55143.
RefSeqi NP_014258.1. NM_001182979.1.

3D structure databases

ProteinModelPortali P53909.
SMRi P53909. Positions 5-321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35686. 142 interactions.
DIPi DIP-4342N.
IntActi P53909. 4 interactions.
MINTi MINT-499127.
STRINGi 4932.YNL141W.

Proteomic databases

MaxQBi P53909.
PaxDbi P53909.
PeptideAtlasi P53909.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL141W ; YNL141W ; YNL141W .
GeneIDi 855581.
KEGGi sce:YNL141W.

Organism-specific databases

CYGDi YNL141w.
SGDi S000005085. AAH1.

Phylogenomic databases

eggNOGi COG1816.
GeneTreei ENSGT00730000111151.
KOi K01488.
OMAi MFRELYS.
OrthoDBi EOG7647CH.

Enzyme and pathway databases

BioCyci YEAST:YNL141W-MONOMER.

Miscellaneous databases

NextBioi 979709.
PROi P53909.

Gene expression databases

Genevestigatori P53909.

Family and domain databases

HAMAPi MF_01962. Adenine_deaminase.
InterProi IPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
[Graphical view ]
Pfami PF00962. A_deaminase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01430. aden_deam. 1 hit.
PROSITEi PS00485. A_DEAMINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Sub-families of alpha/beta barrel enzymes: a new adenine deaminase family."
    Ribard C., Rochet M., Labedan B., Daignan-Fornier B., Alzari P., Scazzocchio C., Oestreicher N.
    J. Mol. Biol. 334:1117-1131(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Adenine deaminase and adenine utilization in Saccharomyces cerevisiae."
    Deeley M.C.
    J. Bacteriol. 174:3102-3110(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Skp1-Cullin-F-box-dependent degradation of Aah1p requires its interaction with the F-box protein Saf1p."
    Escusa S., Laporte D., Massoni A., Boucherie H., Dautant A., Daignan-Fornier B.
    J. Biol. Chem. 282:20097-20103(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SAF1, UBIQUITINATION, MUTAGENESIS OF ILE-69; ASN-72; ASP-219; GLU-237 AND LYS-329.
  9. "Hydrolytic cleavage of N6-substituted adenine derivatives by eukaryotic adenine and adenosine deaminases."
    Pospisilova H., Sebela M., Novak O., Frebort I.
    Biosci. Rep. 28:335-347(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiADE_YEAST
AccessioniPrimary (citable) accession number: P53909
Secondary accession number(s): D6W141
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 20700 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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