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P53909 (ADE_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenine deaminase

Short name=ADE
EC=3.5.4.2
Alternative name(s):
Adenine aminohydrolase
Short name=AAH
Gene names
Name:AAH1
Ordered Locus Names:YNL141W
ORF Names:N1208, N1825
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism. Also exhibits a low activity towards N(6)-substituted adenines that are commonly known as the plant hormones cytokinins. Ref.2 Ref.5 Ref.8 Ref.9

Catalytic activity

Adenine + H2O = hypoxanthine + NH3. HAMAP-Rule MF_03145

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_03145

Subcellular location

Cytoplasm. Nucleus Ref.6.

Induction

Reduced when grown in a poor nitrogen source medium and strongly down-regulated when cells enter quiescence under nutrient-limiting conditions. Ref.5

Post-translational modification

Probably ubiquitinated when cells enter quiescence in response to nutrient limitation, since it is specifically degraded via a process requiring the F-box protein SAF1 and components of the SKP1-Cullin-F-box complex. HAMAP-Rule MF_03145

Miscellaneous

Present with 20700 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=55 µM for adenine Ref.9

pH dependence:

Optimum pH is 7.0.

Temperature dependence:

Optimum temperature is 30-37 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347Adenine deaminase HAMAP-Rule MF_03145
PRO_0000194360

Sites

Active site2071Proton donor By similarity
Metal binding161Zinc; catalytic By similarity
Metal binding181Zinc; catalytic By similarity
Metal binding2041Zinc; catalytic By similarity
Metal binding2851Zinc; catalytic By similarity
Binding site2861Substrate By similarity
Site2281Important for catalytic activity By similarity

Experimental info

Mutagenesis691I → T in aah1-2; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. Ref.8
Mutagenesis721N → K in aah1-3; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. Ref.8
Mutagenesis2191D → G in aah1-4; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. Ref.8
Mutagenesis2371E → V in aah1-6; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. Ref.8
Mutagenesis3291K → E in aah1-7; impairs AAH1 degradation during postdiauxic growth. Ref.8

Sequences

Sequence LengthMass (Da)Tools
P53909 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: E25573A9F9EB7BB6

FASTA34739,635
        10         20         30         40         50         60 
MVSVEFLQEL PKCEHHLHLE GTLEPDLLFP LAKRNDIILP EGFPKSVEEL NEKYKKFRDL 

        70         80         90        100        110        120 
QDFLDYYYIG TNVLISEQDF FDLAWAYFKK VHKQGLVHAE VFYDPQSHTS RGISIETVTK 

       130        140        150        160        170        180 
GFQRACDKAF SEFGITSKLI MCLLRHIEPE ECLKTIEEAT PFIKDGTISA LGLDSAEKPF 

       190        200        210        220        230        240 
PPHLFVECYG KAASLNKDLK LTAHAGEEGP AQFVSDALDL LQVTRIDHGI NSQYDEELLD 

       250        260        270        280        290        300 
RLSRDQTMLT ICPLSNVKLQ VVQSVSELPL QKFLDRDVPF SLNSDDPAYF GGYILDVYTQ 

       310        320        330        340 
VSKDFPHWDH ETWGRIAKNA IKGSWCDDKR KNGLLSRVDE VVTKYSH 

« Hide

References

« Hide 'large scale' references
[1]"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
Mallet L., Bussereau F., Jacquet M.
Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Sub-families of alpha/beta barrel enzymes: a new adenine deaminase family."
Ribard C., Rochet M., Labedan B., Daignan-Fornier B., Alzari P., Scazzocchio C., Oestreicher N.
J. Mol. Biol. 334:1117-1131(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Adenine deaminase and adenine utilization in Saccharomyces cerevisiae."
Deeley M.C.
J. Bacteriol. 174:3102-3110(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Skp1-Cullin-F-box-dependent degradation of Aah1p requires its interaction with the F-box protein Saf1p."
Escusa S., Laporte D., Massoni A., Boucherie H., Dautant A., Daignan-Fornier B.
J. Biol. Chem. 282:20097-20103(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SAF1, UBIQUITINATION, MUTAGENESIS OF ILE-69; ASN-72; ASP-219; GLU-237 AND LYS-329.
[9]"Hydrolytic cleavage of N6-substituted adenine derivatives by eukaryotic adenine and adenosine deaminases."
Pospisilova H., Sebela M., Novak O., Frebort I.
Biosci. Rep. 28:335-347(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46843 Genomic DNA. Translation: CAA86885.1.
Z71417 Genomic DNA. Translation: CAA96024.1.
BK006947 Genomic DNA. Translation: DAA10407.1.
PIRS55143.
RefSeqNP_014258.1. NM_001182979.1.

3D structure databases

ProteinModelPortalP53909.
SMRP53909. Positions 5-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35686. 142 interactions.
DIPDIP-4342N.
IntActP53909. 4 interactions.
MINTMINT-499127.
STRING4932.YNL141W.

Proteomic databases

MaxQBP53909.
PaxDbP53909.
PeptideAtlasP53909.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL141W; YNL141W; YNL141W.
GeneID855581.
KEGGsce:YNL141W.

Organism-specific databases

CYGDYNL141w.
SGDS000005085. AAH1.

Phylogenomic databases

eggNOGCOG1816.
GeneTreeENSGT00730000111151.
KOK01488.
OMAMFRELYS.
OrthoDBEOG7647CH.

Enzyme and pathway databases

BioCycYEAST:YNL141W-MONOMER.

Gene expression databases

GenevestigatorP53909.

Family and domain databases

HAMAPMF_01962. Adenine_deaminase.
InterProIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
[Graphical view]
PfamPF00962. A_deaminase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01430. aden_deam. 1 hit.
PROSITEPS00485. A_DEAMINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979709.
PROP53909.

Entry information

Entry nameADE_YEAST
AccessionPrimary (citable) accession number: P53909
Secondary accession number(s): D6W141
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families