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P53909

- ADE_YEAST

UniProt

P53909 - ADE_YEAST

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Protein

Adenine deaminase

Gene

AAH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism. Also exhibits a low activity towards N(6)-substituted adenines that are commonly known as the plant hormones cytokinins.4 PublicationsUniRule annotation

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Kineticsi

  1. KM=55 µM for adenine1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Temperature dependencei

Optimum temperature is 30-37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Zinc; catalyticUniRule annotation
Metal bindingi18 – 181Zinc; catalyticUniRule annotation
Metal bindingi204 – 2041Zinc; catalyticUniRule annotation
Active sitei207 – 2071Proton donorUniRule annotation
Sitei228 – 2281Important for catalytic activityUniRule annotation
Metal bindingi285 – 2851Zinc; catalyticUniRule annotation
Binding sitei286 – 2861SubstrateUniRule annotation

GO - Molecular functioni

  1. adenine deaminase activity Source: UniProtKB
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. adenine catabolic process Source: UniProtKB
  2. hypoxanthine salvage Source: SGD
  3. nucleotide metabolic process Source: UniProtKB-KW
  4. purine-containing compound salvage Source: SGD
  5. purine ribonucleoside monophosphate biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YNL141W-MONOMER.
ReactomeiREACT_188930. Abacavir metabolism.
REACT_240981. Purine salvage.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine deaminaseUniRule annotation (EC:3.5.4.2UniRule annotation)
Short name:
ADEUniRule annotation
Alternative name(s):
Adenine aminohydrolaseUniRule annotation
Short name:
AAHUniRule annotation
Gene namesi
Name:AAH1UniRule annotation
Ordered Locus Names:YNL141W
ORF Names:N1208, N1825
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL141w.
SGDiS000005085. AAH1.

Subcellular locationi

Cytoplasm 1 PublicationUniRule annotation. Nucleus 1 PublicationUniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691I → T in aah1-2; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication
Mutagenesisi72 – 721N → K in aah1-3; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication
Mutagenesisi219 – 2191D → G in aah1-4; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication
Mutagenesisi237 – 2371E → V in aah1-6; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1. 1 Publication
Mutagenesisi329 – 3291K → E in aah1-7; impairs AAH1 degradation during postdiauxic growth. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Adenine deaminasePRO_0000194360Add
BLAST

Post-translational modificationi

Probably ubiquitinated when cells enter quiescence in response to nutrient limitation, since it is specifically degraded via a process requiring the F-box protein SAF1 and components of the SKP1-Cullin-F-box complex.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiP53909.
PaxDbiP53909.
PeptideAtlasiP53909.

Expressioni

Inductioni

Reduced when grown in a poor nitrogen source medium and strongly down-regulated when cells enter quiescence under nutrient-limiting conditions.1 Publication

Gene expression databases

GenevestigatoriP53909.

Interactioni

Protein-protein interaction databases

BioGridi35686. 143 interactions.
DIPiDIP-4342N.
IntActiP53909. 4 interactions.
MINTiMINT-499127.
STRINGi4932.YNL141W.

Structurei

3D structure databases

ProteinModelPortaliP53909.
SMRiP53909. Positions 5-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1816.
GeneTreeiENSGT00730000111151.
InParanoidiP53909.
KOiK01488.
OMAiMFRELYS.
OrthoDBiEOG7647CH.

Family and domain databases

HAMAPiMF_01962. Adenine_deaminase.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01430. aden_deam. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53909-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVSVEFLQEL PKCEHHLHLE GTLEPDLLFP LAKRNDIILP EGFPKSVEEL
60 70 80 90 100
NEKYKKFRDL QDFLDYYYIG TNVLISEQDF FDLAWAYFKK VHKQGLVHAE
110 120 130 140 150
VFYDPQSHTS RGISIETVTK GFQRACDKAF SEFGITSKLI MCLLRHIEPE
160 170 180 190 200
ECLKTIEEAT PFIKDGTISA LGLDSAEKPF PPHLFVECYG KAASLNKDLK
210 220 230 240 250
LTAHAGEEGP AQFVSDALDL LQVTRIDHGI NSQYDEELLD RLSRDQTMLT
260 270 280 290 300
ICPLSNVKLQ VVQSVSELPL QKFLDRDVPF SLNSDDPAYF GGYILDVYTQ
310 320 330 340
VSKDFPHWDH ETWGRIAKNA IKGSWCDDKR KNGLLSRVDE VVTKYSH
Length:347
Mass (Da):39,635
Last modified:October 1, 1996 - v1
Checksum:iE25573A9F9EB7BB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86885.1.
Z71417 Genomic DNA. Translation: CAA96024.1.
BK006947 Genomic DNA. Translation: DAA10407.1.
PIRiS55143.
RefSeqiNP_014258.1. NM_001182979.1.

Genome annotation databases

EnsemblFungiiYNL141W; YNL141W; YNL141W.
GeneIDi855581.
KEGGisce:YNL141W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46843 Genomic DNA. Translation: CAA86885.1 .
Z71417 Genomic DNA. Translation: CAA96024.1 .
BK006947 Genomic DNA. Translation: DAA10407.1 .
PIRi S55143.
RefSeqi NP_014258.1. NM_001182979.1.

3D structure databases

ProteinModelPortali P53909.
SMRi P53909. Positions 5-321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35686. 143 interactions.
DIPi DIP-4342N.
IntActi P53909. 4 interactions.
MINTi MINT-499127.
STRINGi 4932.YNL141W.

Proteomic databases

MaxQBi P53909.
PaxDbi P53909.
PeptideAtlasi P53909.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL141W ; YNL141W ; YNL141W .
GeneIDi 855581.
KEGGi sce:YNL141W.

Organism-specific databases

CYGDi YNL141w.
SGDi S000005085. AAH1.

Phylogenomic databases

eggNOGi COG1816.
GeneTreei ENSGT00730000111151.
InParanoidi P53909.
KOi K01488.
OMAi MFRELYS.
OrthoDBi EOG7647CH.

Enzyme and pathway databases

BioCyci YEAST:YNL141W-MONOMER.
Reactomei REACT_188930. Abacavir metabolism.
REACT_240981. Purine salvage.

Miscellaneous databases

NextBioi 979709.
PROi P53909.

Gene expression databases

Genevestigatori P53909.

Family and domain databases

HAMAPi MF_01962. Adenine_deaminase.
InterProi IPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
[Graphical view ]
Pfami PF00962. A_deaminase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01430. aden_deam. 1 hit.
PROSITEi PS00485. A_DEAMINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Sub-families of alpha/beta barrel enzymes: a new adenine deaminase family."
    Ribard C., Rochet M., Labedan B., Daignan-Fornier B., Alzari P., Scazzocchio C., Oestreicher N.
    J. Mol. Biol. 334:1117-1131(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Adenine deaminase and adenine utilization in Saccharomyces cerevisiae."
    Deeley M.C.
    J. Bacteriol. 174:3102-3110(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Skp1-Cullin-F-box-dependent degradation of Aah1p requires its interaction with the F-box protein Saf1p."
    Escusa S., Laporte D., Massoni A., Boucherie H., Dautant A., Daignan-Fornier B.
    J. Biol. Chem. 282:20097-20103(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SAF1, UBIQUITINATION, MUTAGENESIS OF ILE-69; ASN-72; ASP-219; GLU-237 AND LYS-329.
  9. "Hydrolytic cleavage of N6-substituted adenine derivatives by eukaryotic adenine and adenosine deaminases."
    Pospisilova H., Sebela M., Novak O., Frebort I.
    Biosci. Rep. 28:335-347(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiADE_YEAST
AccessioniPrimary (citable) accession number: P53909
Secondary accession number(s): D6W141
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 20700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3