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P53738

- TR112_YEAST

UniProt

P53738 - TR112_YEAST

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Protein

Multifunctional methyltransferase subunit TRM112

Gene

TRM112

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase MTQ2, required for the methylation of eRF1 on 'Gln-182'. Together with methyltransferase TRM11, required for the formation of 2-methylguanosine at position 10 (m2G10) in tRNA. Together with methyltransferase BUD23, required for the formation of 7-methylguanine at position 1575 (m7G1575) in 18S rRNA. Involved in biogenesis of both 40S and 60S ribosomal subunits.4 Publications

GO - Biological processi

  1. maturation of LSU-rRNA Source: SGD
  2. maturation of SSU-rRNA Source: SGD
  3. peptidyl-glutamine methylation Source: SGD
  4. ribosomal large subunit biogenesis Source: SGD
  5. ribosomal small subunit biogenesis Source: SGD
  6. rRNA (guanine-N7)-methylation Source: SGD
  7. tRNA methylation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16617.
YEAST:G3O-33353-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional methyltransferase subunit TRM112
Alternative name(s):
eRF1 methyltransferase subunit TRM112
Short name:
eRF1 MTase subunit TRM112
tRNA methyltransferase 112
Gene namesi
Name:TRM112
Ordered Locus Names:YNR046W
ORF Names:N3445
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR046w.
SGDiS000005329. TRM112.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. eRF1 methyltransferase complex Source: SGD
  2. nucleus Source: UniProtKB-KW
  3. tRNA (m2G10) methyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 135135Multifunctional methyltransferase subunit TRM112PRO_0000215805Add
BLAST

Proteomic databases

MaxQBiP53738.
PaxDbiP53738.
PeptideAtlasiP53738.

Expressioni

Gene expression databases

GenevestigatoriP53738.

Interactioni

Subunit structurei

Heterodimer of MTQ2-TRM112, forming the eRF1 methyltransferase. TRM112 is necessary for the solubility and activity of the catalytic subunit MTQ2. Interacts with TRM11; required for full tRNA methyltransferase activity. Interacts with BUD23; required for full rRNA methyltransferase activity. Interacts with RCM1, NOP2, TRM9 and LYS9.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MTQ2Q039204EBI-28520,EBI-31378
TRM11Q124634EBI-28520,EBI-30471

Protein-protein interaction databases

BioGridi35871. 72 interactions.
DIPiDIP-2052N.
IntActiP53738. 7 interactions.
MINTiMINT-546013.
STRINGi4932.YNR046W.

Structurei

Secondary structure

1
135
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 75Combined sources
Helixi14 – 163Combined sources
Beta strandi22 – 243Combined sources
Turni29 – 313Combined sources
Beta strandi33 – 364Combined sources
Helixi44 – 5310Combined sources
Helixi56 – 6510Combined sources
Helixi82 – 843Combined sources
Helixi87 – 10014Combined sources
Beta strandi103 – 1119Combined sources
Turni113 – 1153Combined sources
Beta strandi118 – 1225Combined sources
Beta strandi125 – 1284Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J6AX-ray1.70A1-135[»]
ProteinModelPortaliP53738.
SMRiP53738. Positions 1-135.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53738.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 131130TRM112Add
BLAST

Sequence similaritiesi

Belongs to the TRM112 family.Curated
Contains 1 TRM112 domain.Curated

Phylogenomic databases

eggNOGiNOG241447.
GeneTreeiENSGT00390000009268.
HOGENOMiHOG000181652.
InParanoidiP53738.
KOiK15448.
OMAiHEDEVRH.
OrthoDBiEOG7TQVCM.

Family and domain databases

InterProiIPR005651. UPF0434/Trm112.
[Graphical view]
PfamiPF03966. Trm112p. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53738-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFLTTNFLK CSVKACDTSN DNFPLQYDGS KCQLVQDESI EFNPEFLLNI
60 70 80 90 100
VDRVDWPAVL TVAAELGNNA LPPTKPSFPS SIQELTDDDM AILNDLHTLL
110 120 130
LQTSIAEGEM KCRNCGHIYY IKNGIPNLLL PPHLV
Length:135
Mass (Da):15,066
Last modified:October 1, 1996 - v1
Checksum:i3CFB2ABDDFB0E38A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71661 Genomic DNA. Translation: CAA96327.1.
BK006947 Genomic DNA. Translation: DAA10587.1.
PIRiS63377.
RefSeqiNP_014444.1. NM_001183223.1.

Genome annotation databases

EnsemblFungiiYNR046W; YNR046W; YNR046W.
GeneIDi855782.
KEGGisce:YNR046W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71661 Genomic DNA. Translation: CAA96327.1 .
BK006947 Genomic DNA. Translation: DAA10587.1 .
PIRi S63377.
RefSeqi NP_014444.1. NM_001183223.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2J6A X-ray 1.70 A 1-135 [» ]
ProteinModelPortali P53738.
SMRi P53738. Positions 1-135.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35871. 72 interactions.
DIPi DIP-2052N.
IntActi P53738. 7 interactions.
MINTi MINT-546013.
STRINGi 4932.YNR046W.

Proteomic databases

MaxQBi P53738.
PaxDbi P53738.
PeptideAtlasi P53738.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR046W ; YNR046W ; YNR046W .
GeneIDi 855782.
KEGGi sce:YNR046W.

Organism-specific databases

CYGDi YNR046w.
SGDi S000005329. TRM112.

Phylogenomic databases

eggNOGi NOG241447.
GeneTreei ENSGT00390000009268.
HOGENOMi HOG000181652.
InParanoidi P53738.
KOi K15448.
OMAi HEDEVRH.
OrthoDBi EOG7TQVCM.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16617.
YEAST:G3O-33353-MONOMER.

Miscellaneous databases

EvolutionaryTracei P53738.
NextBioi 980253.
PROi P53738.

Gene expression databases

Genevestigatori P53738.

Family and domain databases

InterProi IPR005651. UPF0434/Trm112.
[Graphical view ]
Pfami PF03966. Trm112p. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA."
    Purushothaman S.K., Bujnicki J.M., Grosjean H., Lapeyre B.
    Mol. Cell. Biol. 25:4359-4370(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRM9; TRM11; LYS9 AND MTQ2.
  6. "The methyltransferase adaptor protein Trm112 is involved in biogenesis of both ribosomal subunits."
    Sardana R., Johnson A.W.
    Mol. Biol. Cell 23:4313-4322(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BUD23; NOP2 AND RCM1.
  7. "Trm112 is required for Bud23-mediated methylation of the 18S rRNA at position G1575."
    Figaro S., Wacheul L., Schillewaert S., Graille M., Huvelle E., Mongeard R., Zorbas C., Lafontaine D.L., Heurgue-Hamard V.
    Mol. Cell. Biol. 32:2254-2267(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BUD23.
  8. "The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast."
    Heurgue-Hamard V., Graille M., Scrima N., Ulryck N., Champ S., van Tilbeurgh H., Buckingham R.H.
    J. Biol. Chem. 281:36140-36148(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS), FUNCTION, INTERACTION WITH MTQ2.

Entry informationi

Entry nameiTR112_YEAST
AccessioniPrimary (citable) accession number: P53738
Secondary accession number(s): D6W1M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3