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P53271 (COG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Conserved oligomeric Golgi complex subunit 2

Short name=COG complex subunit 2
Alternative name(s):
Component of oligomeric Golgi complex 2
Protein SEC35
Gene names
Name:COG2
Synonyms:SEC35
Ordered Locus Names:YGR120C
ORF Names:G6324
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events. COG2 is required for ER to Golgi vesicle docking. Not essential for viability. Ref.4 Ref.7

Subunit structure

Component of the conserved oligomeric Golgi (COG or Sec34/Sec35) complex which consists of eight different proteins COG1-COG8. The COG complex interacts with the Rab GTPase YPT1, the Glogi SNAREs GOS1, SEC22, SED5, VTI1 and YKT6 and the COPI coatomer subunit gamma SEC21. Ref.5 Ref.6 Ref.7 Ref.11

Subcellular location

Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side Ref.4 Ref.9.

Miscellaneous

Present with 3270 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Conserved oligomeric Golgi complex subunit 2
PRO_0000213499

Secondary structure

............... 262
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P53271 [UniParc].

Last modified July 11, 2006. Version 2.
Checksum: 0ED457A7466F0F53

FASTA26230,325
        10         20         30         40         50         60 
MDFLNDDELD LDLPVTAEIS KELFATEIEK YRESETNGTD VDNFDVDRFL VQKNFHYLPL 

        70         80         90        100        110        120 
DSLIRDLSGL SQKMVQTLLE QIRSNYDDYL TFSNTYTDEE NETLINLEKT QSDLQKFMTQ 

       130        140        150        160        170        180 
LDHLIKDDIS NTQEIIKDVL EYLKKLDEIY GSLRNHSQLT EALSLGKRLS KSLHEMCGIE 

       190        200        210        220        230        240 
PLEEEICSGL IEQLYKLITA SRRILESCAD SNSPYIHHLR NDYQDLLQEF QISLKILTEK 

       250        260 
CLENPSSLQN LSLTLVSIIK TA 

« Hide

References

« Hide 'large scale' references
[1]"An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes."
van Dyck L., Tettelin H., Purnelle B., Goffeau A.
Yeast 13:171-176(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Sec35p, a novel peripheral membrane protein, is required for ER to Golgi vesicle docking."
VanRheenen S.M., Cao X., Lupashin V.V., Barlowe C., Waters M.G.
J. Cell Biol. 141:1107-1119(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"The Sec34/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic."
Whyte J.R., Munro S.
Dev. Cell 1:527-537(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[6]"The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins."
Suvorova E.S., Duden R., Lupashin V.V.
J. Cell Biol. 157:631-643(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN COG COMPLEX, SUBUNIT.
[7]"Identification of sec36p, sec37p, and sec38p: components of yeast complex that contains sec34p and sec35p."
Ram R.J., Li B., Kaiser C.A.
Mol. Biol. Cell 13:1484-1500(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE COG COMPLEX.
[8]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"The binary interacting network of the conserved oligomeric Golgi tethering complex."
Loh E., Hong W.
J. Biol. Chem. 279:24640-24648(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF THE COG COMPLEX, INTERACTION WITH COG3 AND COG4.
[12]"Structural analysis of conserved oligomeric Golgi complex subunit 2."
Cavanaugh L.F., Chen X., Richardson B.C., Ungar D., Pelczer I., Rizo J., Hughson F.M.
J. Biol. Chem. 282:23418-23426(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 61-262.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83099 Genomic DNA. Translation: CAA58155.1.
Z72905 Genomic DNA. Translation: CAA97130.1.
BK006941 Genomic DNA. Translation: DAA08212.1.
PIRS55978.
RefSeqNP_011635.2. NM_001181249.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JQQNMR-A61-262[»]
ProteinModelPortalP53271.
SMRP53271. Positions 109-262.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33365. 90 interactions.
DIPDIP-1194N.
IntActP53271. 29 interactions.
MINTMINT-376961.
STRING4932.YGR120C.

Proteomic databases

PaxDbP53271.
PeptideAtlasP53271.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR120C; YGR120C; YGR120C.
GeneID853017.
KEGGsce:YGR120C.

Organism-specific databases

CYGDYGR120c.
SGDS000003352. COG2.

Phylogenomic databases

eggNOGNOG41082.
HOGENOMHOG000111879.
OMARESETNG.
OrthoDBEOG7P5TBF.

Enzyme and pathway databases

BioCycYEAST:G3O-30827-MONOMER.

Gene expression databases

GenevestigatorP53271.

Family and domain databases

InterProIPR024602. COG_su2_N.
[Graphical view]
PfamPF06148. COG2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP53271.
NextBio972884.

Entry information

Entry nameCOG2_YEAST
AccessionPrimary (citable) accession number: P53271
Secondary accession number(s): D6VUQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 11, 2006
Last modified: April 16, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references