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P32897 (TIM23_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM23
Alternative name(s):
Membrane import machinery protein MIM23
Mitochondrial protein import protein 3
Mitochondrial protein import protein MAS6
Gene names
Name:TIM23
Synonyms:MAS6, MIM23, MPI3
Ordered Locus Names:YNR017W
ORF Names:N3180
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Subunit structure

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex. Ref.8

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein. Note: Around 20 amino acids of the N-terminus are believed to span the mitochondrial outer membrane. This association is dynamic and depends on the translocation activity of the TIM23 complex. However, this topology seems not to be critical for formation of a TOM-TIM supercomplex and preprotein import. Ref.9

Miscellaneous

Present with 1440 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Tim17/Tim22/Tim23 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222Mitochondrial import inner membrane translocase subunit TIM23
PRO_0000210307

Regions

Transmembrane1 – ?Helical; Note=Outer membrane; Potential
Topological domain? – 96Mitochondrial intermembrane Potential
Transmembrane97 – 11822Helical; Note=Inner membrane; Potential
Topological domain119 – 14426Mitochondrial matrix Potential
Transmembrane145 – 16622Helical; Note=Inner membrane; Potential
Topological domain167 – 1748Mitochondrial intermembrane Potential
Transmembrane175 – 18915Helical; Note=Inner membrane; Potential
Topological domain190 – 1967Mitochondrial matrix Potential
Transmembrane197 – 21519Helical; Note=Inner membrane; Potential
Topological domain216 – 2227Mitochondrial intermembrane Potential

Sequences

Sequence LengthMass (Da)Tools
P32897 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 08E5AB4F400F675B

FASTA22223,244
        10         20         30         40         50         60 
MSWLFGDKTP TDDANAAVGG QDTTKPKELS LKQSLGFEPN INNIISGPGG MHVDTARLHP 

        70         80         90        100        110        120 
LAGLDKGVEY LDLEEEQLSS LEGSQGLIPS RGWTDDLCYG TGAVYLLGLG IGGFSGMMQG 

       130        140        150        160        170        180 
LQNIPPNSPG KLQLNTVLNH ITKRGPFLGN NAGILALSYN IINSTIDALR GKHDTAGSIG 

       190        200        210        220 
AGALTGALFK SSKGLKPMGY SSAMVAAACA VWCSVKKRLL EK 

« Hide

References

« Hide 'large scale' references
[1]"MAS6 encodes an essential inner membrane component of the yeast mitochondrial protein import pathway."
Emtage J.L.T., Jensen R.E.
J. Cell Biol. 122:1003-1012(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of MIM23, a putative component of the protein import machinery of the mitochondrial inner membrane."
Dekker P.J.T., Keil P., Rassow J., Maarse A.C., Pfanner N., Meijer M.
FEBS Lett. 330:66-70(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site."
Kuebrich M., Keil P., Rassow J., Dekker P.J.T., Blom J., Meijer M., Pfanner N.
FEBS Lett. 349:222-228(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, TOPOLOGY.
[6]"Tim23 links the inner and outer mitochondrial membranes."
Donzeau M., Kaldi K., Adam A., Paschen S., Wanner G., Guiard B., Bauer M.F., Neupert W., Brunner M.
Cell 101:401-412(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
[9]"Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria."
Popov-Celeketic D., Mapa K., Neupert W., Mokranjac D.
EMBO J. 27:1469-1480(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X71633 Genomic DNA. Translation: CAA50640.1.
X74161 Genomic DNA. Translation: CAA52274.1.
Z71632 Genomic DNA. Translation: CAA96296.1.
BK006947 Genomic DNA. Translation: DAA10558.1.
PIRS36139.
RefSeqNP_014414.3. NM_001183194.3.

3D structure databases

ProteinModelPortalP32897.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35842. 50 interactions.
DIPDIP-2451N.
IntActP32897. 14 interactions.
MINTMINT-1359281.
STRING4932.YNR017W.

Chemistry

BindingDBP32897.
ChEMBLCHEMBL1741180.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBP32897.
PaxDbP32897.
PeptideAtlasP32897.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNR017W; YNR017W; YNR017W.
GeneID855751.
KEGGsce:YNR017W.

Organism-specific databases

CYGDYNR017w.
SGDS000005300. TIM23.

Phylogenomic databases

eggNOGCOG5596.
HOGENOMHOG000166435.
KOK17794.
OMAHITKRGP.
OrthoDBEOG75MW7H.

Enzyme and pathway databases

BioCycYEAST:G3O-33332-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).

Gene expression databases

GenevestigatorP32897.

Family and domain databases

InterProIPR003397. Tim17/Tim22/Tim23/PMP24.
IPR005681. Tim23.
[Graphical view]
PfamPF02466. Tim17. 1 hit.
[Graphical view]
TIGRFAMsTIGR00983. 3a0801s02tim23. 1 hit.
ProtoNetSearch...

Other

NextBio980166.
PROP32897.

Entry information

Entry nameTIM23_YEAST
AccessionPrimary (citable) accession number: P32897
Secondary accession number(s): D6W1J2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families