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P00890 (CISY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase, mitochondrial

EC=2.3.3.1
Gene names
Name:CIT1
Synonyms:GLU3, LYS6
Ordered Locus Names:YNR001C
ORF Names:N2019
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subcellular location

Mitochondrion matrix Ref.7.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3737Mitochondrion
Chain38 – 479442Citrate synthase, mitochondrial
PRO_0000005479

Sites

Active site3121 By similarity
Active site3581 By similarity
Active site4131 By similarity

Experimental info

Sequence conflict581E → Q in CAA25359. Ref.1
Sequence conflict781E → EE in CAA25359. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P00890 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 280661B1CB248F14

FASTA47953,360
        10         20         30         40         50         60 
MSAILSTTSK SFLSRGSTRQ CQNMQKALFA LLNARHYSSA SEQTLKERFA EIIPAKAEEI 

        70         80         90        100        110        120 
KKFKKEHGKT VIGEVLLEQA YGGMRGIKGL VWEGSVLDPE EGIRFRGRTI PEIQRELPKA 

       130        140        150        160        170        180 
EGSTEPLPEA LFWLLLTGEI PTDAQVKALS ADLAARSEIP EHVIQLLDSL PKDLHPMAQF 

       190        200        210        220        230        240 
SIAVTALESE SKFAKAYAQG VSKKEYWSYT FEDSLDLLGK LPVIASKIYR NVFKDGKITS 

       250        260        270        280        290        300 
TDPNADYGKN LAQLLGYENK DFIDLMRLYL TIHSDHEGGN VSAHTTHLVG SALSSPYLSL 

       310        320        330        340        350        360 
AAGLNGLAGP LHGRANQEVL EWLFKLREEV KGDYSKETIE KYLWDTLNAG RVVPGYGHAV 

       370        380        390        400        410        420 
LRKTDPRYTA QREFALKHFP DYELFKLVST IYEVAPGVLT KHGKTKNPWP NVDSHSGVLL 

       430        440        450        460        470 
QYYGLTEASF YTVLFGVARA IGVLPQLIID RAVGAPIERP KSFSTEKYKE LVKKIESKN 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method."
Suissa M., Suda K., Schatz G.
EMBO J. 3:1773-1781(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24657 / D273-10B.
[2]"The effect of point mutations in the hinge of yeast citrate synthase."
Lindner P., Plueckthun A.
Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 24657 / D273-10B.
[3]"Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae."
Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.
Yeast 10:523-533(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[4]"Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
Verhasselt P., Aert R., Voet M., Volckaert G.
Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00782 Genomic DNA. Translation: CAA25359.1.
Z23259 Genomic DNA. Translation: CAA80781.1.
X77395 Genomic DNA. Translation: CAA54569.1.
Z71616 Genomic DNA. Translation: CAA96277.1.
BK006947 Genomic DNA. Translation: DAA10543.1.
PIRYKBY. S35390.
RefSeqNP_014398.1. NM_001183178.1.

3D structure databases

ProteinModelPortalP00890.
SMRP00890. Positions 40-478.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35825. 49 interactions.
DIPDIP-4597N.
IntActP00890. 5 interactions.
MINTMINT-567581.
STRING4932.YNR001C.

Proteomic databases

PaxDbP00890.
PeptideAtlasP00890.
PRIDEP00890.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNR001C; YNR001C; YNR001C.
GeneID855732.
KEGGsce:YNR001C.

Organism-specific databases

CYGDYNR001c.
SGDS000005284. CIT1.

Phylogenomic databases

eggNOGCOG0372.
GeneTreeENSGT00750000118689.
HOGENOMHOG000130831.
KOK01647.
OMAELIYEDC.
OrthoDBEOG7WQ82G.

Enzyme and pathway databases

BioCycYEAST:YNR001C-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).
REACT_85873. Metabolism of proteins.
UniPathwayUPA00223; UER00717.

Gene expression databases

GenevestigatorP00890.

Family and domain databases

Gene3D1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01793. cit_synth_euk. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980117.

Entry information

Entry nameCISY1_YEAST
AccessionPrimary (citable) accession number: P00890
Secondary accession number(s): D6W1H7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways